GENETIC CONTROL OF RESTRICTION AND MODIFICATION IN ESCHERICHIA COLI
نویسندگان
چکیده
منابع مشابه
Restriction in genetic crosses between Escherichia coli and Shigella flexneri.
Shigella flexneri restricts Escherichia coli deoxyribonucleic acid (DNA) and can modify phage DNA so that it is restricted in E. coli.
متن کاملGenetics of host-controlled restriction and modification of deoxyribonucleic acid in Escherichia coli.
Lederberg, Seymour (Brown University, Providence, R.I.). Genetics of host-controlled restriction and modification of deoxyribonucleic acid in Escherichia coli. J. Bacteriol. 91:1029-1036. 1966.-The locus for the host specific restriction and modification of deoxyribonucleic acid in Escherichia coli has been mapped by matings between mutants for these characters in strains K-12, C600, and B. Lin...
متن کاملCloning and expression of the Pst I restriction-modification system in Escherichia coli.
Here we report the cloning and preliminary characterization of the Pst I restriction-modification system of Providencia stuartii 164. Transformants of Escherichia coli carrying the Pst I gene system inserted into the cloning vector pBR322 were selected on the basis of acquired resistance to bacteriophage lambda infection. Pst I endonuclease was detected in osmotic shock fluid from each of the r...
متن کاملHost-induced modification-restriction and the utilization of 5-bromouracil by thymineless mutants of Escherichia coli.
An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K.
متن کاملLack of regulation of the modification-dependent restriction enzyme McrBC in Escherichia coli.
Restriction alleviation (RA) by the type I restriction enzyme EcoKI is caused by treatments that damage DNA. RA is due to proteolysis of the EcoKI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1964
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.88.6.1652-1660.1964